Product Overview Documents Bis-ANS is superior to 1-8-ANS as a probe for nonpolar cavities in proteins, often binding with an affinity that is orders-of-magnitude higher. Anilinonaphthalene sulfonates (ANS) are essentially nonfluorescent in water, only becoming appreciably fluorescent when bound to membranes. Depending on context, bis-ANS can both induce LLPS de novo as well as prevent formation of homotypic liquid droplets. Our study also reveals the mechanisms by which bis-ANS and related.
Threedimensional structure of bisANS. For clarity only polar
Bis-ANS is superior to 1-8-ANS as a probe for nonpolar cavities in proteins, often binding with an affinity that is orders-of-magnitude higher. Anilinonaphthalene sulfonates Invitrogen Bis-ANS (4,4'-Dianilino-1,1'-Binaphthyl-5,5'-Disulfonic Acid, | Fisher Scientific 9619517 DOI: 10.1080/07391102.1998.10508216 Abstract Despite emergence of bis-ANS as a major fluorescence probe of proteins structure, conformational and spectroscopic properties of protein/bis-ANS complexes remains largely unexplored. Formal Name 4,4'-bis(phenylamino)- [1,1'-binaphthalene]-5,5'-disulfonic acid, dipotassium salt CAS Number 912-60-7 Synonyms 4,4'-Dianilino-1,1'-binaphthyl-5,5'-disulfonic Acid Molecular Formula C32H22N2O6S2 • 2K Formula Weight 672.9 Purity open access Highlights • Fluorescence-based assay is a fast and easy tool for detection of RNA-IFIT binding. • Bis-ANS probe demonstrates effective binding to the IFIT proteins. • Bis-ANS is located inside the RNA binding tunnel of the IFIT proteins. • Developed assay enables binding studies at low RNA concentrations. Abstract Keywords IFIT
(A) Change of bisANS fluorescence intensity (fluorescence maximum, λ
Bis-ANS exhibits a chaperone-like activity, effectively suppressing protein aggregation and preventing a certain degree of heat-inactivation of enzymes [5]. Horowitz et al. have recently shown that bis-ANS binds to tubulin, inhibiting the formation of microtubules [2], [11]. Their experiments demonstrate the presence of more than one site for. 5,5'-Bis [8- (phenylamino)-1-naphthalenesulfonate] (bis-ANS), the fluorescent probe which binds to tubulin, inhibits its assembly into microtubules [Horowitz et al. (1984) J. Biol. Chem. 259, 14647-14650]. Bis-ANS was the most effective inhibitor of prion peptide aggregation. Bis-ANS binds strongly to rPrP 23-231 leading to a substantial increase in -sheet content and to limited oligomerization. Because ATP and ATP analogs partially displace complexed bis-ANS it has also been possible to study interactions of S-1 and nucleotides by using the displacement effect. Approximate values of the parameters of these various interactions have been measured. Some possible applications of bis-ANS have been explored.
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compound Summary 4,4'-Dianilino-1,1'-binaphthyl-5,5'-disulfonic acid dipotassium salt PubChem CID 16213473 Structure Chemical Safety Laboratory Chemical Safety Summary (LCSS) Datasheet Molecular Formula C32H22K2N2O6S2 Synonyms Bis-ans 65664-81-5 4,4'-Dianilino-1,1'-binaphthyl-5,5'-disulfonic acid dipotassium salt Bis-ANS (dipotassium) These results resemble effects of bis-ANS on BSA, where low ratios of bis-ANS:protein were found to increase thermostability of BSA, while higher ratios induced transition of the protein into a molten globule-like state (Celej et al. 2003). Therefore, we propose that accelerated aggregation of IL-1ra in the presence of 4.2-21 mM ANS reflects.
bis-ANS 1,1′-bi (4-anilino)naphtalene-5,5′-disulfonic acid α-Crystallin, a major lens protein, plays an important role in maintaining the transparency and refractive properties of the eye lens ( 1., 2., 3. ). The lenticular α-crystallin consists of two 20-kDa polypeptide chains, αA and αB, that share about 60% sequence homology. bis-ANS is a high-affinity non-covalent extrinsic fluorescent dye used to analyze protein conformation.1 Its predominant interaction with proteins is through its hydrophobic phenyl and naphthyl rings.2 bis-ANS has an excitation maxima of 390 nm.3 It has an emission maximum of 523 nm when free in solution but
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Bis-ANS is a fluorescent probe for nonpolar cavities in proteins. Used to detect A β fibre (K d = ~80 nM); exhibits distinct fluorescent profiles for fibres and oligomers. Fluoresces in hydrophobic environments, negligible fluorescence in water solutions. Excitation\emission λ ~ 355\520 nm at pH 7.4. Also modulates liquid-liquid phase. Bis-ANS is a fluorescent probe for nonpolar cavities in proteins. Used to detect A β fibre (K d = ~80 nM); exhibits distinct fluorescent profiles for fibres and oligomers. Fluoresces in hydrophobic environments, negligible fluorescence in water solutions. Excitation\emission λ ~ 355\520 nm at pH 7.4.
